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Conformational landscapes are formed of much more than open and closed states. And yet, we often base biological research on a few static structures. Biomolecular mechanisms are ruled by dynamics - rapid interconversions between states, structural heterogeneity and transient intermediates drive function, expanding far beyond open and closed snapshots. See how Howard et al.'s research revealed two previously unknown intermediate states of the bacterial helicase Rep, demonstrating the vital role of adding single-molecule dynamics to crystal structures > https://lnkd.in/euSJWBm3

Is the missing piece of your understanding of structure-function relationships an unobserved conformation? High-resolution structures provide invaluable insights into biomolecular architecture, but important functional states can remain hidden between known conformations. Discover how, starting from atomic structures, Doxsey and Shen used single-molecule FRET to determine the relative populations of different states present in dynamic equilibrium under different conditions to elucidate the detailed mechanism of their system > https://lnkd.in/eqXgisyE

Could a hidden conformational state be the missing piece in your mechanistic studies? Mechanistic insight often depends on resolving the transient states that link molecular recognition to functional output. See how Streit et al. combined single-molecule measurements, cryo-EM, and biochemical assays to resolve the dynamic conformational ensemble of the flaviviral RNA stem-loop A. Their work shows how RNA dynamics direct a post-binding structural search that converts an initial encounter with the NS5 polymerase into a functional replication-initiation complex > https://lnkd.in/enEAcGKW

Is the missing piece of your understanding of structure-function relationships an unobserved conformation? High-resolution structures provide invaluable insights into biomolecular architecture, but important functional states can remain hidden between known conformations. Discover how, starting from atomic structures, Doxsey and Shen used single-molecule FRET to determine the relative populations of different states present in dynamic equilibrium under different conditions to elucidate the detailed mechanism of their system > https://lnkd.in/e5hs8pQ9

Conformational landscapes are formed of much more than open and closed states. And yet, we often base biological research on a few static structures. Biomolecular mechanisms are ruled by dynamics - rapid interconversions between states, structural heterogeneity and transient intermediates drive function, expanding far beyond open and closed snapshots. See how Howard et al.'s research revealed two previously unknown intermediate states of the bacterial helicase Rep, demonstrating the vital role of adding single-molecule dynamics to crystal structures > https://lnkd.in/e-Sw6FdS

Could a hidden conformational state be the missing piece in your mechanistic studies? Mechanistic insight often depends on resolving the transient states that link molecular recognition to functional output. See how Streit et al. combined single-molecule measurements, cryo-EM, and biochemical assays to resolve the dynamic conformational ensemble of the flaviviral RNA stem-loop A. Their work shows how RNA dynamics direct a post-binding structural search that converts an initial encounter with the NS5 polymerase into a functional replication-initiation complex > https://lnkd.in/eVk4G9wG