Waters Corporation

Are your highly polar analytes eluting too early? Highly polar compounds can be difficult to retain with conventional reversed-phase LC, increasing the risk of poor separation, matrix interference, and unreliable quantitation. Explore practical strategies for improving the retention and separation of highly polar compounds using HILIC, mixed-mode chromatography, and derivatization approaches. Download the eBook for food and beverage testing.

Reversed-phase, HILIC, mixed-mode, or derivatization? Selecting the right chromatographic approach is often one of the biggest challenges in polar analyte method development. Different techniques provide retention through different mechanisms and offer distinct advantages depending on the analyte and application. Learn practical considerations for selecting and optimizing chromatographic approaches for food, beverage, and environmental testing. Download the eBook for food and beverage testing.

Struggling to retain highly polar analytes? Poor retention remains one of the most common challenges in the analysis of highly polar compounds by liquid chromatography. Early elution can lead to co-elution, matrix interference, and reduced confidence in quantitative results. Explore practical strategies for improving retention and selectivity using reversed-phase, HILIC, mixed-mode, and derivatization approaches. Download the eBook for food, beverage, and environmental testing

Analyzing highly polar compounds can be challenging. Poor retention, limited selectivity, and matrix interference can all impact method performance. Swipe to explore common chromatographic challenges and practical considerations for method development.

Bringing GLP-1s and other synthetic peptides to market requires clear, reliable impurity analysis. When conventional columns fail to resolve closely eluting impurities, teams face never-ending method optimization, costly rework, and greater regulatory scrutiny. In this on-demand webinar, Waters scientists show how new reversed-phase analytical tools can improve the separation of GLP-1 receptor agonist and insulin impurities. Learn how this approach can deliver up to 2x higher resolution and up to 3x faster separations, so you can spend less time optimizing methods and move peptide therapeutic development forward with confidence.

Evaluating wash and elution conditions can make SPE method development time-consuming. Discover how the 20 Bottle Approach supports systematic screening, more efficient optimization, and robust bioanalytical methods with Oasis™ HLB.